5-Ethynyl Uridine,5-EU,69075-42-9
5-Ethynyl uridine (5-EU) is a metabolic labeling reagent used to detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide using two-step technique.
In step one, the alkyne-containing uridine analog 5-EU is fed to cells or animals and actively incorporated into RNA. In step two the incorporated 5-EU is detected via chemoselective ligation or “click” reaction between an azide and an alkyne where the modified biomolecules are detected with a corresponding azide-containing dye or hapten. Cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis (HCS).
Molecular weight
268.22
Chemical composition
C11H12N2O6
CAS
69075-42-9
Solubility
Water, DMSO, DMF
Purity
>95% (HPLC)
Appearance
Off white to grey crystalline
Storage conditions
-20C
Shipping conditions
Ambient temperature
5-Ethynyl Uridine,5-EU,69075-42-9
公司：西安瑞禧生物科技有限公司