5-TAMRA Azide (also known as TAMRA azide, isomer 5) is the red-fluorescent probe that is patible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free &lduo;click chemistry&rduo; reaction to form a stable triazole and does not reuire Cu-catalyst or elevated temperatures.
Although the mixed isomers of 5(6)-TAMRA azide is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often reuired in the conjugation processes.
5-TAMRA azide is exact replacement of Invitrogen&rsuo;s (Thermo Fisher Scientific) Tetramethylrhodamine (TAMRA) Azide (Tetramethylrhodamine 5-Carboxamido-(6-Azidohexanyl)), 5-isomer
Abs/Em Maxima
553/575
Extinction coefficient
92,000
Flow cytometry laser line
532 or 555 nm
Microscopy laser line
532 or 555 nm
Spectrally similar dyes
Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
Molecular weight
555.67
CAS
1006592-61-5
Solubility
Water, DMSO, DMF, MeOH
Purity
>98% (HPLC)
Appearance
Red solid
Storage conditions
-20C
Shipping conditions
Ambient temperature
5-TAMRA Azide|5-羧甲基罗丹明叠氮
其他染料列表：
5(6)-Carboxy tetramethyl rhodamine;5(6)-TAMRA 150347-56-1
5-Carboxytetramethylrhodamine; 5-TAMRA 91809-66-4
6-Carboxytetramethylrhodamine;6-TAMRA 91809-67-5
5(6)-Carboxytetramethylrhodamine succinimidyl ester;5(6)-TAMRA, SE 246256-50-8
5-Carboxytetramethylrhodamine succinimidyl ester;5-TAMRA, SE 150810-68-7
6-Carboxytetramethylrhodamine succinimidyl ester;6-TAMRA, SE 150810-69-8
1,4-dichloro 5-Carboxytetramethylrhodamine 198546-47-3
1,4-dichloro 6-Carboxytetramethylrhodamine 407581-83-3
5-Carboxy-X- rhodamine; 5-ROX 216699-35-3
6-Carboxy-X- rhodamine; 6-ROX 194785-18-7
5(6)-Carboxy-X- rhodamine; 5(6)-ROX 198978-94-8
5(6)-ROX, SE [5-(6)-Carboxy-X-rhodamine, succinimidyl ester]
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester]
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]
Rhodamine 101;Rhodamine 640 64339-18-0
Rhodamine 110 chloride 13558-31-1
Rhodamine 123 62669-70-9
6-ROX C2 Maleimide
6-ROX alkyne
6-ROX azide
5(6)-TAMRA Maleimide
5-TAMRA Maleimide
6-TAMRA Maleimide
5-TAMRA alkyne
6-TAMRA alkyne
5-TAMRA azide
6-TAMRA azide
5(6)-TAMRA cadaverine
5-TAMRA cadaverine
6-TAMRA cadaverine
5(6)-TAMRA ethylenediamine
5-TAMRA ethylenediamine
6-TAMRA ethylenediamine
6-Aminotetramethylrhodamine (6-氨基四甲基罗丹明) 80724-18-1
5-Aminotetramethylrhodamine (5-氨基四甲基罗丹明) 80724-17-0,167095-10-5
5-TAMRA cadaverine
5-TAMRA NHS Ester 150810-68-7
5(6)-TAMRA NHS Ester 150408-83-6
TAMRA Acid 98181-63-6
5-Carboxy Fluorescein; 5-FAM 5-羧基荧光素 76823-03-5
6-Carboxy Fluorescein; 6-FAM 6-羧基荧光素 3301-79-9
5(6)Carboxy fluorescein; 5(6)-FAM 5(6)羧基荧光素72088-94-9
1,4-dichloro 5-Carboxytetramethylrhodamine 198546-47-3
1,4-dichloro 6-Carboxytetramethylrhodamine 407581-83-3
Sulforhodamine 101; Sulforhodamine 640 60311-02-6
5-Carboxy-X-rhodamine; 5-ROX 216699-35-3
6-Carboxy-X-rhodamine; 6-ROX 194785-18-7
5(6)-Carboxy-X-rhodamine; 5(6)-ROX 198978-94-8
Rhodamine 101;Rhodamine 640 64339-18-0
Cy2 260430-02-2
Cy2-SE;Cy2 NHS Ester 1205-33-4
Cy3 146368-13-0
Cy3-SE;Cy3 NHS Ester 146368-16-3
Cy5 146368-11-8
Cy5-SE;Cy5 NHS Ester 146368-14-1
Cy7 943298-08-6
Cy7-SE; Cy7 NHS Ester 477908-53-5
Cy3-alkyne 10103-62-5
Cy5-alkyne 1345823-20-2
Cy7-alkyne
Calcein tetraethyl ester 1170856-93-5
Dansyl chloride丹磺酰氯 605-65-2
Dansyl acid 6268-49-1
DABCYL acid, SE 146998-31-4
DNP-X acid 10466-72-5
8-Hydroxy julolidine 41175-50-2
3,6-Dichloro trimellitic acid 137071-78-4
Boc-1,2-diaminoethane 57260-73-8
Boc-1,3-diaminobutane 75178-96-0
Boc-1,4-diaminobutane 68076-36-8
N-(4-Bromobutyl)phthalimide 5394-18-3
2-[2-[2-(2-Bromoethoxy)ethoxy]ethoxy]-ethanol 85141-94-2
14-bromo-3,6,9,12-tetraoxatetradecan-1-ol 957205-14-0
Diethyl carboxymethylphosphonate 3095-95-2
Indocyanine Green ICG吲哚菁绿 3599-32-4
PI（碘化丙啶） Propidium Iodide 25535-16-4
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