5-TAMRA Azide (also known as TAMRA azide, isomer 5) is the red-fluorescent probe that is patible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free &lduo;click chemistry&rduo; reaction to form a stable triazole and does not reuire Cu-catalyst or elevated temperatures.
Although the mixed isomers of 5(6)-TAMRA azide is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often reuired in the conjugation processes.
5-TAMRA azide is exact replacement of Invitrogen&rsuo;s (Thermo Fisher Scientific) Tetramethylrhodamine (TAMRA) Azide (Tetramethylrhodamine 5-Carboxamido-(6-Azidohexanyl)), 5-isomer
Abs/Em Maxima
553/575
Extinction coefficient
92,000
Flow cytometry laser line
532 or 555 nm
Microscopy laser line
532 or 555 nm
Spectrally similar dyes
Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
Molecular weight
555.67
CAS
1006592-61-5
Solubility
Water, DMSO, DMF, MeOH
Purity
>98% (HPLC)
Appearance
Red solid
Storage conditions
-20C
Shipping conditions
Ambient temperature
5-TAMRA Azide|5-TAMRA N3|1006592-61-5
其他罗丹明荧光染料试剂：
5(6)-Carboxy tetramethyl rhodamine;5(6)-TAMRA 150347-56-1
5-Carboxytetramethylrhodamine; 5-TAMRA 91809-66-4
6-Carboxytetramethylrhodamine;6-TAMRA91809-67-5
&uot;5(6)-Carboxytetramethylrhodamine succinimidyl ester;5(6)-TAMRA, SE&uot; 246256-50-8
&uot;5-Carboxytetramethylrhodamine succinimidyl ester;5-TAMRA, SE&uot; 150810-68-7
&uot;6-Carboxytetramethylrhodamine succinimidyl ester;6-TAMRA, SE&uot; 150810-69-8
&uot;1,4-dichloro 5-Carboxytetramethylrhodamine&uot; 198546-47-3
&uot;1,4-dichloro 6-Carboxytetramethylrhodamine&uot; 407581-83-3
Sulforhodamine 101; Sulforhodamine 640 60311-02-6
5-Carboxy-X- rhodamine; 5-ROX 216699-35-3
6-Carboxy-X- rhodamine; 6-ROX 194785-18-7
5(6)-Carboxy-X- rhodamine; 5(6)-ROX 198978-94-8
6 d ROX
5 d ROX
Rhodamine 101;Rhodamine 640 64339-18-0
5(6)-TAMRA C6 maleimide
5-TAMRA C6 maleimide
6-TAMRA C6 maleimide
5(6)-TAMRA Maleimide
5-TAMRA Maleimide
6-TAMRA Maleimide
5-TAMRA alkyne
6-TAMRA alkyne
5-TAMRA azide
6-TAMRA azide
5(6)-TAMRA cadaverine
5-TAMRA cadaverine
6-TAMRA cadaverine
5(6)-TAMRA ethylenediamine
5-TAMRA ethylenediamine
6-TAMRA ethylenediamine
5-TAMRA TEA Salt (5-羧基四甲基罗丹明三乙胺盐) 150322-05-7
5-TAMRA-NHS (5-羧基四甲基罗丹明琥珀酰亚胺酯) 3212-17-3
6-TAMRA TEA salt (6-羧基四甲基罗丹明三乙胺盐) 150322-06-8
6-Aminotetramethylrhodamine (6-氨基四甲基罗丹明) 80724-18-1
5-Aminotetramethylrhodamine (5-氨基四甲基罗丹明) 80724-17-0,167095-10-5
6-FAM-C6-OH 6-(N-6-羟基己基)酰胺荧光素 467456-19-5
5(6)-FAM-C6-OH 5/6-(N-6-羟基己基)酰胺荧光素 9889-37-2；467456-19-5
Fmoc-Lys(5-FAM)-OH FAM标记的Lys赖氨酸 1242933-88-5
Fmoc-Lys(5/6-FAM)-OH 5/6-FAM标记的Lys赖氨酸 CAS: 1242933-88-5；1266666-04-9)
5(6)-TET 5/6-羧基四氯荧光素 155911-14-1
5(6)-HEX 5/6-羧基六氯荧光素 155911-16-3
4-Aminomethyl-6-FAM (6-CAM ) 4-氨甲基-6-羧基荧光素 326802-08-8
4-Aminomethyl-5-FAM (5-CAM ) 4-氨甲基-5-羧基荧光素 198546-45-1
Fura-2 ethyl ester 96331-97-4
BAPTA tetraethyl ester 73630-07-6
Calcein 207124-64-9
Boc-Statine 58521-49-6
Fmoc-Statine 158257-40-0
Diacetate-5-FAM-NHS 5-羧基二乙酰化荧光素琥珀酰亚胺酯 150206-05-6
Diacetate-6-FAM-NHS 6-羧基二乙酰化荧光素琥珀酰亚胺酯 150206-15-8
6-Carboxy-X-Rhodamine，SE (NHS) 6-羧基-X-罗丹明琥珀酰亚胺酯 216699-36-4
5-Carboxy-X-Rhodamine，SE (NHS) 5-羧基-X-罗丹明琥珀酰亚胺酯 209734-74-7
Dipiv-5-FAM-Osu (NHS) 5-羧基-二特戊酰氧基荧光素琥珀酰亚胺酯
Dipiv-5-FAM 6-羧基荧光素二特戊酸酯
Dipiv-5/6-FAM-C6-OH 5/6-N-(6-羟基己基)酰胺-荧光素二特戊酸酯
ATTO 565 meta-isomer(1+)
ATTO 565 para-isomer(1+)
Fmoc-Glu(EDANS)-OH 193475-66-0
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