Labeling of cysteine residues, as well as other thiolated molecules
Cy3.5 maleimide can be used for selective labeling of free sulfhydryl groups (e.g. cysteine residues in peptides, proteins and oligonucleotides). Before labeling, cystines should be prepared with reductant such as TCEP (tris-carboxyethylphosphine). The mono-reactive malemides is particularly good for the labeling of antibody, that’s because the dye molecules are located away from the antibody binding site and functioning of the labeled molecules can be protected.
化学名
3-(6-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)ethyl)amino)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E)-3-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)prop-1-en-1-yl)-1H-benzo[e]indol-3-ium chloride
Canonical SMILES
CN1C2=CC=C3C=CC=CC3=C2C(C)(C)/C1=C\C=C\C4=[N+](CCCCCC(NCCN5C(C=CC5=O)=O)=O)C6=CC=C(C=CC=C7)C7=C6C4(C)C.[Cl-]
分子式
C44H47ClN4O3
分子量
715.32
溶解度
≥ 71.5mg/mL in DMSO, ≥ 28.5 mg/mL in EtOH with ultrasonic
储存条件
Store at -20°C
General tips
For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition
Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request