Labeling of amino-groups in biomolecules.
Cyanine3.5 NHS ester is a dye for labeling of oligonucleotides, peptides and proteins which contain amino-groups. For fluorescence imaging and other fluorescence-based biochemical analysis, it has been used to label biological molecules too. Cy3.5 dyes have enhanced fluorescence upon binding to proteins. The excitation peak and emission peak of Cy3.5 dye is around 550nm and 590nm respectively. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

别名
N/A

化学名
2-((1E,3E)-3-(3-butyl-1,1-dimethyl-1H-benzo[e]indol-2(3H)-ylidene)prop-1-en-1-yl)-3-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-1,1-dimethyl-1H-benzo[e]indol-3-ium

Canonical SMILES
CC1(C)C2=C3C(C=CC=C3)=CC=C2[N+](CCCCCC(ON4C(CCC4=O)=O)=O)=C1/C=C/C=C5C(C)(C)C6=C(C=CC=C7)C7=CC=C6N\5CCCC

分子式
C42H44ClN3O4
分子量
690.27
溶解度
≥ 69mg/mL in DMSO, ≥ 7.29 mg/mL in EtOH with ultrasonic
储存条件
-20°C in the dark.

General tips
For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.

Shipping Condition
Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request