Ethoxy or methoxyl silane functionalized polyethylene glycol, silane PEG (PEG-Si) is a surface reactive PEG derivative that can be used to modify glass, silica and other surfaces via the reaction between hydroxyl group and ethoxyl/methoxyl silane. Pegylation can greatly suppress the non-specific binding of charged molecules to the modified surfaces. And they have wide applications for medica device, biomems or biocompatible material development. Physical Properties: Off-white/white solid or viscous liquid depends on molecule weight; Soluble in regular aqeous solution as well as most organic solvents; Storage Conditions: Store at -20 0C, desiccated. Silane PEG tends to hydrolyze from moisture. Avoid frequent thaw and frozen. Reaction Procedures: Generally, silane PEG is first dissolved in a mixture of ethanol/water solution, then allow the solution to contact with oxidized glass or silica surface for 30 min to 2 hours. Under this condition, silane PEG should bind to the material covalently. Materials Required: Pegylation solution: Ethanol/water (w/w, 95%/5%); Silane PEG stock solution, 10~50 mg/mL in pegylation solution, prepare in fresh. Washing solution: Distilled water. Reaction Steps: 1. Dissolve targeted materials in Pegylation buffer. 2. Add silane PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of silane PEG needed for optimal conjugation; 3. Allow mixture agitates at room temperature for 30 min at room temperature or 2 hours at 4 -20 0C. 4. Wash out unreacted materials either by distilled water.
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