50多种常用荧光染料的激发及发射波长
荧光染料

Ex（nm）

Em（nm）
FITC

490

525
TRITC

547

572
Cy2

489

506
Cy3

555

570
Cy5

646

620
Cy5.5

675

694
Cy7

750

773
Calcein

494

517
Rhodamine 110

496

520
Rhodamine B

555

580
5-Carboxy Fluorescein

492

518
5-FAM, SE

492

520
6-TAMRA SE

543

575
6-Carboxy-X-rhodamine

568

590
Alexa TM 488

490

520
BODIPY

530

550
Alexa TM 546

555

570
Pyronin Y

550

580
Thiadicarbocyanine

651

670
Alexa TM 594

590

615
6-Aminofluorescein

490

520
Dansyl chloride

337

492
Rhodamine 123

507

529
4-Methylumbelliferone

385

502
5-carboxy-2,7-dichlorofluorescein

504

529
5-Carboxynapthofluorescein

512

568
5-Carboxytetramethylrhodamine (5-TAMRA)

542

568
5-FAM (5-Carboxyfluorescein)

492

518
5-ROX (carboxy-X-rhodamine)

567

591
5-TAMRA (5-Carboxytetramethylrhodamine)

548

552
Acriflavin

436

520
Alexa Fluor 350™

346

442
Alexa Fluor 430™

431

540
Alexa Fluor 488™

492

520
Alexa Fluor 532™

531

553
Alexa Fluor 568™

577

603
Alexa Fluor 633™

632

650
Alexa Fluor 680™

679

702
AMCA（Aminomethylcoumarin）

345

425
Aminocoumarin

346

442
Anthrocyl stearate

381

446
APC-Cy7

655

755
Atabrine

436

490
Aurophosphine

490

515
Bisbenzamide

360

461
Bodipy 492/515

490

515
Bodipy 500/510

500

510
Bodipy 530/550

528

547
Bodipy 576/589

579

590
Catecholamine

410

470
Coumarin Phalloidin

387

470
Dansyl Amine

337

517
Dansyl Chloride

372

518
Hydroxycoumarin

325

386
NBD

466

539
PerCP

488

675
Nile Red

556

590
Noradrenaline

340

490
Thiolyte

385

488
Merocyanin

555

578
BODIPY TMR

542

568
 
西安瑞禧生物科技有限公司
Xi'an ruixi Biological Technology Co.,Ltd