5-TAMRA Picolyl Azide,Alexa Fluor 546
产品介绍
5-TAMRA Picolyl Azide
5-TAMRA Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concen*ration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concen*ration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold. In addition, the use of 5-TAMRA Picolyl Azide instead of conventional 5-TAMRA Azide allows for at least a tenfold reduction in the concen*ration of the copper catalyst without sacrificing the efficiency of labeling.
Although the mixed isomers of 5(6)-TAMRA is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often reuired in the conjugation processes.
In summary, the in*roduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.
Abs/Em Maxima

545/576
Extinction coefficient

91,000
Flow cytometry laser line

532 or 555 nm
Microscopy laser line

532 or 555 nm
Spectrally similar dyes

Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
Molecular weight

808.89
CAS

N/A
Solubility

DMSO, DMF, MeOH
Purity

>97% (HPLC)
Appearance

Red solid
Storage conditions

-20C
Shipping conditions

Ambient temperature
结构式：
5-TAMRA Picolyl Azide,Alexa Fluor 546
西安瑞禧生物科技有限公司
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炔烃 Dye alkynes
1-Ethynyl pyrene
3-Ethynyl perylene
BDP FL alkyne
FAM alkyne, 5-isomer
FAM alkyne, 6-isomer
TAMRA alkyne, 5-isomer
BDP TMR alkyne
Cyanine3 alkyne
Cyanine5 alkyne
Cyanine5.5 alkyne
Cyanine7 alkyne
Cyanine7.5 alkyne
Sulfo-Cyanine3 alkyne
Sulfo-Cyanine5 alkyne