Cy5® hydrazide,CY5酰肼染料
Cyanine5 hydrazide is a reactive dye for the labeling of aldehydes and ketones, an analog of Cy5® hydrazide.
This dye reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. Examples are proteins subjected to oxidative stress, glycosylated proteins pre-activated by periodate oxidation (including antobodies), and oligonucleotides with aldehyde moieties.
Cyanine5 hydrazide replaces carbonyl-reactive Cy5®, Alexa Fluor 647, DyLight 649 dyes.
Cy5® hydrazide,CY5酰肼染料
General properties
Appearance:dark blue powder
Molecular weight:569.61
Molecular formula:C32H42Cl2N4O
Solubility:moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Quality control:NMR 1H, HPLC-MS (95%)
Storage conditions:Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Spectral properties
Excitation maximum, nm:646
ε, L⋅mol−1⋅cm−1:250000
Emission maximum, nm:662
Fluorescence quantum yield:0.2
CF260:0.03
CF280:0.04