FAM NHS ester, 6-isomer，羧基荧光染料
Fluorescein (FAM) is a bright fluorophore that has found an extremely wide application in life science research. The fluorophore is hydrophilic and its derivatives possess good solubility in aqueous buffers.
Fluorescein is compatible with a wide variety of fluorescent instrumentation - most instruments are equipped with FAM channel filters.
This product contains the pure 6-isomer of fluorescein.
General properties：
Appearance: | yellow crystalline solid |
Mass spec M+ increment: | 358.0 |
Molecular weight: | 473.39 |
Molecular formula: | C25NO9H15 |
IUPAC name: | (2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate |
Solubility: | good in DMF, DMSO |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
FAM NHS ester, 6-isomer，羧基荧光染料
Spectral properties：
Excitation maximum, nm: | 494 |
ε, L⋅mol−1⋅cm−1: | 75000 |
Emission maximum, nm: | 520 |
Fluorescence quantum yield: | 0.9 |
CF260: | 0.20 |
CF280: | 0.17 |
