Cy3 Tetrazine|Cyanine3 Tetrazine|Cy3四嗪
Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics are desired, a faster hydrogen substituted Cy®3 Tetrazine probe would be a probe of choice.
 
Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes.
 
Cy®Dye is a trademark of GE Healthcare.
structural formula:         
Cy3 Tetrazine|Cyanine3 Tetrazine|Cy3四嗪
 
Abs/Em Maxima
550/570 nm
Extinction coefficient
150,000
Flow cytometry laser line
532 or 555 nm
Microscopy laser line
532 or 555 nm
Spectrally similar dyes
Alexa Fluor® 555, Atto™ 555, CF™ 555 Dye, DyLight™555
Molecular weight
894.05
CAS
N/A
Solubility
Water, DMSO, DMF
Purity
>90% (HPLC)
Appearance
Red solid
Storage conditions
-20C
Shipping conditions
Ambient temperature