used for Click chemistry to label alkyne-modified oligonucleotides
Cy3.5 azide is a solid material dye ready for the use in Click Chemistry reaction. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
Cas No.
SDF
化学名
3-(6-((3-azidopropyl)amino)-6-oxohexyl)-2-((1E,3E)-3-(3-butyl-1,1-dimethyl-1H-benzo[e]indol-2(3H)-ylidene)prop-1-en-1-yl)-1,1-dimethyl-1H-benzo[e]indol-3-ium
Canonical SMILES
CC1(C)C2=C3C(C=CC=C3)=CC=C2[N+](CCCCCC(NCCCN=[N+]=[N-])=O)=C1/C=C/C=C4C(C)(C)C5=C(C=CC=C6)C6=CC=C5N\4CCCC
分子式
C44H53ClN6O
分子量
717.38
溶解度
≥ 71.7mg/mL in DMSO, ≥ 44.2 mg/mL in EtOH with ultrasonic
储存条件
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
General tips
For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition
Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request