used for Click chemistry to label any molecule bearing azide group.
Cy5 alkyne, with low aqueous solubility is a dye ready for the use in Click Chemistry reaction. Various molecules via Click Chemistry reaction can be attached by this deeply colored, and photostable Cy5 fluorophore. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Various substrates such as biomolecules, polymers, and solid surfaces which bearing azides can be used for the labeling.
别名
N/A
化学名
3,3-dimethyl-1-(6-oxo-6-(prop-2-yn-1-ylamino)hexyl)-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium chloride
Canonical SMILES
CC(C1=CC=CC=C1N/2C)(C)C2=C/C=C/C=C/C3=[N+](CCCCCC(NCC#C)=O)C(C=CC=C4)=C4C3(C)C.[Cl-]
分子式
C35H42ClN3O
分子量
556.18
溶解度
≥ 55.6mg/mL in DMSO, ≥ 50 mg/mL in EtOH with ultrasonic
储存条件
Store at -20°C
General tips
For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition
Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request