Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. Labeled proteins thus obtained are used in NIR bioimaging applications. In tissues or in live organism, NIR imaging systems can be used to visualize distribution of labeled proteins. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. In splenocytes, for ease of detection, P22 particles were labeled internally with Cy7-maleimide. P22-Cy7 alone or P22-Cy7 decorated with DecSelf was incubated with splenocytes at 37℃ to encourage phagocytosis [1].Reference:[1]. Schwarz, B.; Madden, P.; Avera, J.; Gordon, B.; Larson, K.; Miettinen, H.; Uchida, M.; LaFrance, B.; Basu, G.; Rynda-Apple, A.; Douglas, T. Symmetry Controlled, Genetic Presentation of Bio-Active Proteins on the P22 Virus-Like Particle Using an External Decoration Protein. ACS Nano, 2015, 9(9), 9134–9147.
Cy7 maleimide is a near-infrared, sulfhydryl reactive dye which has low aqueous solubility. This reagent can label the proteins contain free sulfhydryl groups. Labeled proteins thus obtained are used in NIR bioimaging applications. In tissues or in live organism, NIR imaging systems can be used to visualize distribution of labeled proteins. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
In splenocytes, for ease of detection, P22 particles were labeled internally with Cy7-maleimide. P22-Cy7 alone or P22-Cy7 decorated with DecSelf was incubated with splenocytes at 37℃ to encourage phagocytosis [1].
Reference:
[1]. Schwarz, B.; Madden, P.; Avera, J.; Gordon, B.; Larson, K.; Miettinen, H.; Uchida, M.; LaFrance, B.; Basu, G.; Rynda-Apple, A.; Douglas, T. Symmetry Controlled, Genetic Presentation of Bio-Active Proteins on the P22 Virus-Like Particle Using an External Decoration Protein. ACS Nano, 2015, 9(9), 9134–9147.
Cas No.
SDF
化学名
6-[(2E)-3,3-dimethyl-2-[(2E)-2-[3-[(E)-2-(1,3,3-trimethylindol-1-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]indol-1-yl]-N-[2-(2,5-dioxopyrrol-1-yl)ethyl]hexanamide
Canonical SMILES
CC1(C2=CC=CC=C2[N+](=C1C=CC3=CC(=CC=C4C(C5=CC=CC=C5N4CCCCCC(=O)NCCN6C(=O)C=CC6=O)(C)C)CCC3)C)C
分子式
C43H51ClN4O3
分子量
707.34
溶解度
soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
储存条件
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
General tips
For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition
Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
