Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. This regent is especially useful for the labeling of proteins which denaturate in the presence of organic co-solvents, as well as for proteins with low solubility. Sulfo-Cy5 NHS ester is one of the most popular fluorophores. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye-dye interactions.
Sulfo-Cy5 NHS ester, was conjugated to LLP2A with 646/662 peak excitation/emission in dimethyl sulfoxide. LLP2A exhibited punctate staining around the cell periphery, consistent with it labeling VLA-4 [1].
Reference:[1] Soodgupta, D. ; Zhou, H.; Beaino, W.; Lu, L.; Rettig, M.; Snee, M.; Skeath, J.; DiPersio, J.F.; Akers, W.J.; Laforest, R.; Anderson, C.J.; Tomasson, M.H.; Shokeen, M. Ex Vivo and In Vivo Evaluation of Overexpressed VLA-4 in Multiple Myeloma Using LLP2A Imaging Agents. Journal of Nuclear Medicine, 2016, 57(4), 640–645.
Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. This regent is especially useful for the labeling of proteins which denaturate in the presence of organic co-solvents, as well as for proteins with low solubility. Sulfo-Cy5 NHS ester is one of the most popular fluorophores. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye-dye interactions.
Sulfo-Cy5 NHS ester, was conjugated to LLP2A with 646/662 peak excitation/emission in dimethyl sulfoxide. LLP2A exhibited punctate staining around the cell periphery, consistent with it labeling VLA-4 [1].
Reference:
[1] Soodgupta, D. ; Zhou, H.; Beaino, W.; Lu, L.; Rettig, M.; Snee, M.; Skeath, J.; DiPersio, J.F.; Akers, W.J.; Laforest, R.; Anderson, C.J.; Tomasson, M.H.; Shokeen, M. Ex Vivo and In Vivo Evaluation of Overexpressed VLA-4 in Multiple Myeloma Using LLP2A Imaging Agents. Journal of Nuclear Medicine, 2016, 57(4), 640–645.
Cas No.
N/A
SDF
别名
N/A
化学名
(2E)-1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate
Canonical SMILES
CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=CC=C3C(C4=C(N3CCCCCC(=O)ON5C(=O)CCC5=O)C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C
分子式
C36H40N3NaO10S2
分子量
761.84
溶解度
very good in water, good in DMF and DMSO
储存条件
Store at -20°C, protect from light
General tips
For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition
Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
