Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions. Sulfo-Cy5 azide is not only a sulfonated but also a popular fluorophore. For labeling proteins and peptides, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.In mammalian cells, Sulfo-Cy5-azide was incubated with U2OS cells. Finally, the study only find that the cells expressing tRNAIle2-5S fusion RNA can be labeled and imaged with Sulfo-Cy5-azide [1]. Reference:[1] Li, F. ; Dong, J.; Hu, X.; Gong, W.; Li, J.; Shen, J.; Tian, H.; Wang, J. A Covalent Approach for Site-Specific RNA Labeling in Mammalian Cells. Angewandte Chemie International Edition, 2015, 54(15), 4597–4602.
Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions. Sulfo-Cy5 azide is not only a sulfonated but also a popular fluorophore. For labeling proteins and peptides, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.
In mammalian cells, Sulfo-Cy5-azide was incubated with U2OS cells. Finally, the study only find that the cells expressing tRNAIle2-5S fusion RNA can be labeled and imaged with Sulfo-Cy5-azide [1].
Reference:
[1] Li, F. ; Dong, J.; Hu, X.; Gong, W.; Li, J.; Shen, J.; Tian, H.; Wang, J. A Covalent Approach for Site-Specific RNA Labeling in Mammalian Cells. Angewandte Chemie International Edition, 2015, 54(15), 4597–4602.
Cas No.
N/A
SDF
别名
N/A
化学名
(2E)-1-[6-(3-azidopropylamino)-6-oxohexyl]-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate
Canonical SMILES
CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=CC=C3C(C4=C(N3CCCCCC(=O)NCCCN=[N+]=[N-])C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C
分子式
C36H47N6NaO8S2
分子量
778.91
溶解度
very high in water
储存条件
Store at -20°C in the dark
General tips
For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition
Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
