carbonyl-reactive dye
Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage. Hydrazide-containing Cyanine Dyes can react with glycoproteins and other glycoconjugates specifically via their carbohydrate groups without interfering binding site or active centers. Glycoproteins also can be labeled after periodate oxidation to form aldehyde groups. Therefore, this dye is especially applicable for the labeling of antibody.
Moreover, Cy5.5 is a near infrared fluorophore with prominent ability of tissue penetration, so it is suitable for the applications which require low fluorescence background, and for the in vivo NIR imaging. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
Cy5.5 hydrazide is a reactive dye for the labeling of biomolecules containing carbonyl groups, such as ketone and aldehydes. This dye pocesses a hydrazide group that can be used to label glycans at their reducing end or other reducing sugars, forming a hydrazone linkage. Hydrazide-containing Cyanine Dyes can react with glycoproteins and other glycoconjugates specifically via their carbohydrate groups without interfering binding site or active centers. Glycoproteins also can be labeled after periodate oxidation to form aldehyde groups. Therefore, this dye is especially applicable for the labeling of antibody.
Moreover, Cy5.5 is a near infrared fluorophore with prominent ability of tissue penetration, so it is suitable for the applications which require low fluorescence background, and for the in vivo NIR imaging. For biomolecule labeling, the labeling regent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
Cas No.
N/A
SDF
别名
N/A
化学名
3-(6-hydrazinyl-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)penta-1,3-dien-1-yl)-1H-benzo[e]indol-3-ium chloride hydrochloride
Canonical SMILES
CN1C2=CC=C3C=CC=CC3=C2C(C)(C)/C1=C\C=C\C=C\C4=[N+](CCCCCC(NN)=O)C5=CC=C(C=CC=C6)C6=C5C4(C)C.[Cl-].Cl
分子式
C40H46Cl2N4O
分子量
669.73
溶解度
≥ 67mg/mL in DMSO, ≥ 98.4 mg/mL in EtOH
储存条件
Store at -20°C
General tips
For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition
Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request