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Cy5 Picolyl Azide,Cy5-N3,Alexa Fluor 647

作者:Cy5 Picolyl Azide,Cy5-N3,Alexa Fluor 647 发布时间:2022-09-23 14:30:52 次浏览

Cy5 Picolyl Azide,Cy5-N3,Alexa Fluor 647

产品介绍

Cy5 Picolyl Azide

Cy5 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concen*ration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concen*ration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold.

In addition, the use of Cy5 Picolyl Azide instead of conventional Cy5-Azide allows for at least a tenfold reduction in the concen*ration of the copper catalyst without sacrificing the efficiency of labeling.

In summary, the in*roduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.

Abs/Em Maxima

647/663 nm

Extinction coefficient

250,000

Flow cytometry laser line

633 or 635 nm

Microscopy laser line

633 or 635 nm

Spectrally similar dyes

Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649

Molecular weight

953.15

CAS

N/A

Solubility

Water, DMSO, DMF, MeOH

Purity

>95% (HPLC)

Appearance

Blue solid

Storage conditions

-20C

Shipping conditions

Ambient temperature

结构式:

 Cy5 Picolyl Azide,Cy5-N3,Alexa Fluor 647(图1)

Cy5 Picolyl Azide,Cy5-N3,Alexa Fluor 647

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