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Coumarin 343 X NHS ester

作者:Coumarin 343 X NHS ester 发布时间:2022-09-20 14:20:48 次浏览

Coumarin 343 X NHS ester

Blue emitting Coumarin 343 dye can form a FRET pair with fluorescein (FAM). An amine reactive form, activated NHS ester.

This amine-reactive NHS ester contains an aminohexanoyl linker between the fluorophore, and the reactive group. This linker provides better solubility and spatial separation between the fluorophore, and the target molecule being labeled.

This reagent can be used to design FRET based assays with FAM as acceptor, and to construct systems which harvest blue light energy.

      Coumarin 343 X NHS ester(图1)

 

General properties:

Appearance:

yellow solid

Molecular weight:

495.52

Molecular formula:

C26H29N3O7

Solubility:

good in DCM, DMSO, DMF

Quality control:

NMR 1H, HPLC-MS (95%)

Storage conditions:

Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

 

Spectral properties:

Excitation maximum, nm:

437

ε, Lmol1⋅cm1:

39000

Emission maximum, nm:

477

Fluorescence quantum yield:

0.63

CF260:

0.29

CF280:

0.24

Coumarin 343 X NHS ester

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