FAM azide| FAM N3|FAM叠氮化物
FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.
With versatility of Click chemistry and this reagent, it is possible to attach this popular fluorophore to nearly any alkyne bearing molecule.
FAM azide is available both as solid compound, and as 10 mM solution in DMSO ready to use in our recommended labeling protocol. This product is a pure 5-isomer. FAM is a replacement for Alexa Fluor 488, DyLight 488.
FAM azide| FAM N3|FAM叠氮化物
General properties：
Appearance: | yellowish crystals |
Molecular weight: | 458.42 |
Molecular formula: | C24H18N4O6 |
Solubility: | soluble in polar organic solvents (DMF, DMSO, alcohols) |
Quality control: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
Spectral properties：
Excitation maximum, nm: | 494 |
ε, L⋅mol−1⋅cm−1: | 75000 |
Emission maximum, nm: | 520 |
Fluorescence quantum yield: | 0.9 |
CF260: | 0.20 |
CF280: | 0.17
|
荧光染料示例 | 激发光 |
Indo-1, Ca saturated | 331 nm |
Indo-1 Ca2+ | 346 nm |
Cascade Blue BSA pH 7.0 | 401 nm |
Cascade Blue | 398 nm |
LysoTracker Blue | 373 nm |
Alexa 405 | 401 nm |
LysoSensor Blue pH 5.0 | 374 nm |
LysoSensor Blue | 374 nm |
DyLight 405 | 399 nm |
DyLight 350 | 332 nm |
BFP (Blue Fluorescent Protein) | 380 nm |
Alexa 350 | 343 nm |
7-Amino-4-methylcoumarin pH 7.0 | 346 nm |
Amino Coumarin | 345 nm |
AMCA conjugate | 347 nm |
Coumarin | 360 nm |
7-Hydroxy-4-methylcoumarin | 360 nm |
7-Hydroxy-4-methylcoumarin pH 9.0 | 361 nm |
6,8-Difluoro-7-hydroxy-4-methylcoumarin pH 9.0 | 358 nm |
Hoechst 33342 | 352 nm |
Pacific Blue | 404 nm |
Hoechst 33258 | 352 nm |
Hoechst 33258-DNA | 352 nm |
Pacific Blue antibody conjugate pH 8.0 | 404 nm |
PO-PRO-1 | 434 nm |
PO-PRO-1-DNA | 435 nm |
POPO-1 | 433 nm |
POPO-1-DNA | 433 nm |
DAPI-DNA | 359 nm |
DAPI | 358 nm |
Marina Blue | 362 nm |
SYTOX Blue-DNA | 445 nm |
CFP (Cyan Fluorescent Protein) | 434 nm |
eCFP (Enhanced Cyan Fluorescent Protein) | 437 nm |
1-Anilinonaphthalene-8-sulfonic acid (1,8-ANS) | 375 nm |
Indo-1, Ca free | 346 nm |
1,8-ANS (1-Anilinonaphthalene-8-sulfonic acid) | 375 nm |
BO-PRO-1-DNA | 462 nm |
BOPRO-1 | 462 nm |
BOBO-1-DNA | 461 nm |
SYTO 45-DNA | 451 nm |
evoglow-Pp1 | 448 nm |
evoglow-Bs1 | 448 nm |
evoglow-Bs2 | 448 nm |
Auramine O | 431 nm |
DiO | 487 nm |
LysoSensor Green pH 5.0 | 447 nm |
Cy 2 | 489 nm |
LysoSensor Green | 447 nm |
Fura-2, high Ca | 336 nm |
Fura-2 Ca2+sup> | 336 nm |
SYTO 13-DNA | 488 nm |
YO-PRO-1-DNA | 491 nm |
YOYO-1-DNA | 491 nm |
eGFP (Enhanced Green Fluorescent Protein) | 488 nm |
LysoTracker Green | 503 nm |
GFP (S65T) | 489 nm |
BODIPY FL, MeOH | 502 nm |
Sapphire | 396 nm |
BODIPY FL conjugate | 503 nm |
MitoTracker Green | 490 nm |
MitoTracker Green FM, MeOH | 490 nm |
Fluorescein 0.1 M NaOH | 493 nm |
Calcein pH 9.0 | 494 nm |
Fluorescein pH 9.0 | 490 nm |
Calcein | 493 nm |
Fura-2, no Ca | 367 nm |
Fluo-4 | 494 nm |
FDA | 495 nm |
DTAF | 495 nm |
Fluorescein | 495 nm |
Fluorescein antibody conjugate pH 8.0 | 493 nm |
CFDA | 495 nm |
FITC | 495 nm |
Alexa Fluor 488 hydrazide-water | 493 nm |
DyLight 488 | 493 nm |
5-FAM pH 9.0 | 492 nm |
FITC antibody conjugate pH 8.0 | 495 nm |
Alexa 488 | 493 nm |
Rhodamine 110 | 497 nm |
Rhodamine 110 pH 7.0 | 497 nm |
Acridine Orange | 431 nm |
Alexa Fluor 488 antibody conjugate pH 8.0 | 499 nm |
BCECF pH 5.5 | 485 nm |
PicoGreendsDNA quantitation reagent | 502 nm |
SYBR Green I | 498 nm |
Rhodaminen Green pH 7.0 | 497 nm |
CyQUANT GR-DNA | 502 nm |
NeuroTrace 500/525, green fluorescent Nissl stain-RNA | 497 nm |
DansylCadaverine | 335 nm |
Rhodol Green antibody conjugate pH 8.0 | 499 nm |
Fluoro-Emerald | 495 nm |
Nissl | 497 nm |
Fluorescein dextran pH 8.0 | 501 nm |
Rhodamine Green | 497 nm |
5-(and-6)-Carboxy-2', 7'-dichlorofluorescein pH 9.0 | 504 nm |
DansylCadaverine, MeOH | 335 nm |
eYFP (Enhanced Yellow Fluorescent Protein) | 514 nm |
Oregon Green 488 | 498 nm |
Oregon Green 488 antibody conjugate pH 8.0 | 498 nm |
Fluo-3 | 506 nm |
BCECF pH 9.0 | 501 nm |
SBFI-Na+ | 336 nm |
Fluo-3 Ca2+ | 506 nm |
Rhodamine 123, MeOH | 507 nm |
FlAsH | 509 nm |
Calcium Green-1 Ca2+ | 506 nm |
Magnesium Green | 507 nm |
DM-NERF pH 4.0 | 493 nm |
Calcium Green | 506 nm |
Citrine | 515 nm |
LysoSensor Yellow pH 9.0 | 335 nm |
TO-PRO-1-DNA | 515 nm |
Magnesium Green Mg2+ | 507 nm |
Sodium Green Na+ | 507 nm |
TOTO-1-DNA | 514 nm |
Oregon Green 514 | 512 nm |
Oregon Green 514 antibody conjugate pH 8.0 | 513 nm |
NBD-X | 466 nm |
DM-NERF pH 7.0 | 509 nm |
NBD-X, MeOH | 467 nm |
CI-NERF pH 6.0 | 513 nm |
Alexa 430<
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