FAM hydrazide,5-isomer|FAM羧基荧光素酰肼
Many natural and synthetic molecules contain aldehyde or ketone carbonyl groups. These carbonyls react with hydrazides with the formation of hydrazones. The reaction is spontaneous at pH values around neutral, and the resulting hydrazones are very stable.
Compounds containing 1,2-diol function, like sugars, can be oxidized with sodium periodate with the formation of carbonyl compounds for the subsequent modification with hydrazides. This is an efficient method for the labeling of glycoproteins (like antibodies), and polysaccharides.
FAM hydrazide is a hydrazide label for the attachment of fluorescein, a bright dye for 488 nm channel, to carbonyl compounds.
FAM hydrazide,5-isomer|FAM羧基荧光素酰肼
General properties：
Appearance: | yellow solid |
Mass spec M+ increment: | 372.07 |
Molecular weight: | 426.81 |
Molecular formula: | C21H15N2ClO6 |
Solubility: | good in ethanol, DMF, DMSO |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties：
Excitation maximum, nm: | 494 |
ε, L⋅mol−1⋅cm−1: | 75000 |
Emission maximum, nm: | 520 |
Fluorescence quantum yield: | 0.9 |
CF260: | 0.20 |
CF280: | 0.17 |
酰肼 Dye hydrazides Pyrene hydrazide BDP FL hydrazide Cyanine3 hydrazide Cyanine5 Boc-hydrazide Cyanine5 hydrazide Cyanine5.5 hydrazide Cyanine7 hydrazide Cyanine7.5 hydrazide 马来酰亚胺 Dye maleimides BDP FL maleimide Cyanine3 maleimide Cyanine5 maleimide Cyanine5.5 maleimide Cyanine7 maleimide Cyanine7.5 maleimide Sulfo-Cyanine3 maleimide Sulfo-Cyanine5 maleimide 羧酸 Carboxylic acids Pyrenebutyric acid BDP FL carboxylic acid Cyanine3 carboxylic acid Cyanine3.5 carboxylic acid Cyanine5 carboxylic acid Cyanine5.5 carboxylic acid Cyanine7 carboxylic acid Cyanine7.5 carboxylic acid Sulfo-Cyanine3 carboxylic acid Sulfo-Cyanine5 carboxylic acid Sulfo-Cyanine7 carboxylic acid 点击化学试剂 非荧光炔烃 Non-fluorescent alkynes Pentynoic acid STP ester Biotin alkyne 非荧光叠氮化物 Non-fluorescent azides Azidobutyric acid NHS ester Biotin azide 寡核苷酸分析 亚磷酰胺 Phosphoramidites FAM Phosphoramidite, 6-Isomer Alkyne Amidite, hydroxyprolinol Alkyne Phosphoramidite, 5'-terminal DMS(O)MT aminolink C6 Long trebler phosphoramidite Pyrene phosphoramidite dU PCR/qPCR PCR/qPCR ROX reference dye for qPCR SYBR Green I Gel Staining Solution, 10000x SYBR Green I for Real Time PCR, 100x CPG固相修饰 CPG solid supports Alkyne CPG modifier 500 TFA-Amino modifier CPG 500 三磷酸酯修饰 Modified triphosphates Ami1-ddUTP Ami1-dUTP |
