分子量 897.25 分子式 N/A CAS N/A 溶(解)度 Water, DMSO, DMF 纯度 >95% (HPLC) 外观 Red solid 储存环境 -20°C. Desiccate 运输条件 Ambient temperatureAbs/Em Maxima: 578/602 nm Extinction Coefficient: 88,000 Flow Cytometry Laser Line: 532 nm or 568 nm Microscopy Laser Line: 532 nm or 568 nm Spectrally Similar Dyes: Alexa Fluor® 568, CF® 568 AZDye™ 568 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References). In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol. In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging. AZDye 568™ (Alexa Fluor® 568 equivalent) is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. AZDye™ 568 dye structurally is identical to Alexa Fluor® 568 Dye.