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AFDye 647 Azide

作者:AFDye 647 Azide 发布时间:2022-09-05 17:14:11 次浏览

分子量 955.14 (protonated) 分子式 N/A CAS N/A 溶(解)度 Water, DMSO, DMF 纯度 >95% (HPLC) 外观 Blue solid 储存环境 -20°C. Desiccate 运输条件 Ambient temperatureAbs/Em Maxima: 648/671 nm Extinction Coefficient: 270,000 Flow Cytometry Laser Line: 633 nm or 647 nm Microscopy Laser Line: 633 nm or 647 nm Spectrally Similar Dyes: Alexa Fluor® 647, CF® 647, DyLight® 649 AZDye™ 647 Azide (formerly sold under AFDye™ 647 Azide label) is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe. Its excitation ideally suited for the 633 nm or 647 nm laser lines. AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AZDye™ 647 Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or AZDye™ 647 over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. AZDye™ 647 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets. AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AZDye™ 647 Azide can be used a less expensive alternative to Alexa Fluor® 647 Azide.