分子量 1049.22 (protonated) 分子式 N/A CAS N/A 溶(解)度 Water, DMSO, DMF 纯度 >95% (HPLC) 外观 Red solid 储存环境 -20°C. Desiccate 运输条件 Ambient temperatureAbs/Em Maxima: 555/572 nm Extinction Coefficient: 155,000 Flow Cytometry Laser Line: 532 nm or 555 nm Microscopy Laser Line: 532 nm or 555 nm Spectrally Similar Dyes: Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye AZDye™ 555 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References). In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol. In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging. AZDye™ 555 Picolyl Azide is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes.