分子量 816.82 分子式 N/A CAS N/A 溶(解)度 Water, DMSO, DMF 纯度 >95% (HPLC) 外观 Orange to light red solid 储存环境 -20°C. Desiccate 运输条件 Ambient temperatureAbs/Em Maxima: 532/554 nm Extinction Coefficient: 78,000 Flow Cytometry Laser Line: 532 nm Microscopy Laser Line: 532 nm Spectrally Similar Dyes: Alexa Fluor® 532, Atto™ 532, CF® 532 dyes AZDye™ 532 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References). In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol. In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging. AZDye™ 532 is structurally identical to Alexa Fluor® 532. Its absorption/emission spectra is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye.