英文名：ICG maleimide
     ICG-MAL
中文名：吲哚菁绿-马来酰亚胺
CAS：2143933-81-5
分子式: C51H56N4O6S
分子量: 853.09
外 观：绿色粉末
溶解度：二氯甲烷
纯 度：95%+
ICG-MAL
供应商：瑞禧生物
The maleimide of the ICG dye offers the opportunity to develop optimal conjugates. Maleimide active group provides an efficient and convenient way to selectively link ICG dye to sulfhydryl groups (free thiol, R-SH) on various substrates (antibodies[1], peptides, proteins, oligonucleotides, small molecule drugs etc.) at neutral (physiological) pH without any activation. Maleimides have very low reactivity with amines, alcohols, and phenols (incl. tyrosine and histidine) and do not react with histidine and methionine thus providing very high labeling selectivity.
It has been reported that conjugation to some antibodies may cause decrease in fluorescence intensity of ICG bound to antibody due to the formation of H-dimers4. However, fluorescence is restored after cell binding and internalization, which can be used to design an activatable NIR fluorescent probe. Fluorescence intensity of the unbound ICG-antibody conjugate can be dramatically increased (up to 50-fold) by addition of SDS and β-mercaptoethanol, which diminishes π- π stacking5.
ICG NHS ester的NHS可以和蛋白、多肽或抗体上面的NH2发生反应链接到一起。
ICG-maleimide的马来酰亚胺可以和蛋白、多肽、小分子或抗体上的SH巯基反应连接。
ICG-Amine的NH2可以和蛋白、多肽小分子上面的NHS反应连接起来。
ICG和PEG链接可在体内长循环。
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