Coumarin 343 X azide|Coumarin 343 X N3
Coumarin 343 is a blue emitting fluorophore with an emission maximum around 480 nm. This dye forms a FRET pair with fluorescein, and can harvest blue light energy for the subsequent transfer to other fluorophores.
The azide derivative can be conjugated with alkynes in a copper-catalyzed and copper-free Click chemistry reactions. The molecule contains a long aminohexanoyl linker that provides separation between the dye, and the azide function.
Coumarin 343 X azide|Coumarin 343 X N3
 
General properties:Appearance:yellow solid
Mass spec M+ increment:480.3
Molecular weight:480.56
Molecular formula:C25H32N6O4
IUPAC name:5-{[6-(3-Azidopropylamino)-6-oxohexylamino]carbonyl}-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-4-one
Solubility:good in DMF, DMSO
Quality control:NMR 1H, HPLC-MS (95%)
Storage conditions:Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
 
Spectral properties:Excitation maximum, nm:437
ε, L⋅mol−1⋅cm−1:39000
Emission maximum, nm:477
Fluorescence quantum yield:0.63
CF260:0.29
CF280:0.24
 
产品列表:Coumarin 343 X NHS ester        Ex/Em:430nm-470nm
Coumarin NHS ester              Ex/Em:400nm-450nm
Coumarin NHS ester              Ex/Em:450nm-490nm
Coumarin NHS ester              Ex/Em:470nm-510nm
 
Coumarin 343 X azide            Ex/Em:430nm-470nm
Coumarin azide                  Ex/Em:490nm-530nm
Coumarin azide                  Ex/Em:480nm-520nm
 
Coumarin 343 X alkyne           Ex/Em:430nm-470nm
Coumarin alkyne                 Ex/Em:410nm-460nm
 
Coumarin 343 X maleimide        Ex/Em:430nm-470nm
Coumarin maleimide              Ex/Em:440nm-480nm
 
Coumarin 343 X carboxylic acid  Ex/Em:430nm-470nm
Coumarin carboxylic acid        Ex/Em:440nm-480nm
 
Coumarin 343 X amine            Ex/Em:430nm-470nm
Coumarin amine                  Ex/Em:440nm-480nm
 
Coumarin 343 X Thiol            Ex/Em:430nm-470nm
Coumarin Thiol                  Ex/Em:440nm-480nm