名称
FITC-PEG-DBCO
基本信息：
中文名称：Dibenzocyclooctyne-聚乙二醇-荧光素 (FITC-PEG-DBCO)
别名：FITC-PEG-DBCO
英文名称：FITC-PEG-DBCO，Fluorescein-PEG-DBCO
CAS：-
分子量：400、600、1K、2K、3.4K、4K、5K、10K
纯度：90%以上
应用简介：
DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction.  This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.
Copper-free Click Reaction Procedures:
Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.
物理性质：
状态：淡黄色到黄色固体，或者半固体，取决于分子量。
溶解性：溶于DMSO,DMF等溶剂。
保持条件：-20℃长期保存，避光，干燥。
注意事项：取用一定要干燥，避免频繁的溶解和冻干。
反应性：DBCO于含有N3基团的反应。
技术指标：
纯度（PEG）：90%以上
取代率（DBCO）：90%以上
取代率（FITC）：90%以上
分散系数（PDI）：小于等于1.05
分析：COA、GPC、NMR、MALDI-TOF