Cy3 Picolyl Azide|Cy3吡啶甲基叠氮
Cy3 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concentration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold.
In addition, the use of Cy3 Picolyl Azide instead of conventional Cy3-Azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling.
In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.
structural formula:        
Cy3 Picolyl Azide|Cy3吡啶甲基叠氮
 
Abs/Em Maxima
550/570 nm
Extinction coefficient
150,000
Flow cytometry laser line
532 555 or 568 nm
Microscopy laser line
532 or 555 nm
Spectrally similar dyes
Alexa Fluor® 555, Atto™ 555, CF™ 555 Dye, DyLight™549
Molecular weight
908.08
CAS
N/A
Solubility
Water, DMSO, DMF, MeOH
Purity
>95% (HPLC)
Appearance
Red solid
Storage conditions
-20C
Shipping conditions
Ambient temperature
 
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分子量：544.64
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分子量：452.50
Pyrene azide 1 分子式：C23H22N4O3
分子量：402.45
Pyrene azide 2 分子式：C24H24N4O3
分子量：416.47
Pyrene azide 3 分子式：C26H28N4O3
分子量：444.53
R110 azide, 5- isomer 分子式：C24H20N6O4
分子量：456.45
EXmax：496nm
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EXmax：548nm
EMmax：563nm
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分子量：778.91
EXmax：646nm
EMmax：662nm
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分子量：512.56
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分子量：713.84
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分子量：409.5