BDP FL tetrazine,氟化硼二吡咯-四嗪 Inverse electron demand Diels-Alder reaction (ied-DA) with tetrazines is a promising tool for the conjugation of biomolecules. The reaction takes place between tetrazine as an electron acceptor heterodiene, and a strained dienophile, such as trans-cyclooctene, cyclopropene, or some cyclooctynes. Methyltetrazine provides greater stability in buffers than unsubstituted tetrazine. The rate of its reaction with cycloalkenes still beats almost all other conjugation reaction rates by a factor of magnitudes. BDP FL is a bright dye for fluorescein (FAM) channel. Using BDP FL tetrazine, the dye can be conjugated with molecules bearing strained olefins. General properties Appearance: red crystalline solid Mass spec M+ increment: 447.2 Molecular weight: 475.3 Molecular formula: C24H24N7BF2O Solubility: good in DCM, DMSO, DMF Quality control: NMR 1H, HPLC-MS (95%) Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. Spectral properties Excitation maximum, nm: 503 ε, L⋅mol−1⋅cm−1: 80000 Emission maximum, nm: 509 Fluorescence quantum yield: 0.97 CF260: 0.015 CF280: 0.027 BDP FL tetrazine,氟化硼二吡咯-四嗪 马来酰亚胺 Dye maleimides Pyrene maleimide BDP FL maleimide Cyanine3 maleimide Cyanine5 maleimide Cyanine5.5 maleimide Cyanine7 maleimide Cyanine7.5 maleimide Sulfo-Cyanine3 maleimide Sulfo-Cyanine5 maleimide 伯胺 Primary amine BDP FL amine Cyanine3 amine Cyanine5 amine Cyanine5.5 amine Cyanine7 amine Cyanine7.5 amine Sulfo-Cyanine5 amine Sulfo-Cyanine7 amine 酰肼 Dye hydrazides Pyrene hydrazide BDP FL hydrazide Cyanine3 hydrazide Cyanine5 Boc-hydrazide Cyanine5 hydrazide Cyanine5.5 hydrazide Cyanine7 hydrazide Cyanine7.5 hydrazide